壳聚糖酶

本品是专一性酶制剂，与其它壳聚糖降解方法相比，具有无副反应、降解条件温和、降解过程及降解产物相对分子量分布容易控制。酶解产物活性高，纯度高等优势。

一 、壳聚糖溶液的配制

可聚糖原料溶于3%-5%醋酸溶液，配比成10%的壳聚糖溶液。

二、壳聚糖酶规格、使用方法

本公司提供的活力单位为8000单位/克酶制剂。

三、酶制剂的酶活力规定、注意事项

1.本酶制剂采用单位时间内底物的减少量的方法规定酶活力的单位。

2.酶活力定义：在45℃ pH5.5-6 条件下，每分钟转化1毫克的壳聚糖为水溶性组分所需要的酶量为一个活力单位。

3. 酶解反应制备壳寡糖的原料是壳聚糖，壳聚糖的分子量（黏度）和脱乙酰度，对于酶解反应有很大的影响，同样的酶制剂使用不同的壳聚糖作为底物，测出的酶活力不尽相同。因此在使用本酶制剂时，用使用的壳聚糖原料自行测定酶活力，以便计算酶制剂的使用量。

Compared with other degradation methods of chitosan, this product has no side reaction, mild degradation conditions, degradation process and molecular weight distribution of degradation products are easy to control. The enzyme hydrolysate has the advantages of high activity and high purity. The raw material of chitosan solution is dissolved in 3% -5% acetic acid solution, and the ratio is 10% chitosan solution. 2. chitosanase specification and method of use the active unit provided by our company is 8000 units/gram enzyme preparation. Weight enzymatic hydrolysis time enzymatic hydrolysis condition 1 kg 3 g 4 H Temperature: 45 ° C PH: PH 5.5-6 enzymatic hydrolysis reaction process should be stirred slowly, keep the internal temperature of the container even 45 ° C, avoid high speed stirring, avoid inactivation of enzyme preparation. Regulation of enzyme activity of enzyme preparation and points for attention 1. The enzyme preparation defines the units of enzyme activity by reducing the amount of substrate per unit time. 2. Enzyme activity definition: the amount of enzyme required to convert 1 mg of chitosan to a water-soluble component per minute is a unit of activity at 45 ° C PH 5.5-6. 3. The raw materials of Chitooligosaccharides are chitosan. The molecular weight (viscosity) and degree of deacetylation of chitosan have great influence on the enzymatic hydrolysis, the enzyme activity was different. Therefore, when the enzyme preparation is used, the enzyme activity is determined by the chitosan raw material used so as to calculate the amount of the enzyme preparation used.