葡萄糖氧化酶是从特异青霉（Penicillium notatum）等霉菌和蜂蜜中发现的酶。它能催化D-葡萄糖+O2D-葡糖酸（的δ-内酯）+H2O2的反应。EC1．1．3．4．特异青霉（P．notatum）的酶以其显有抗菌性而引入注意。因此，也有葡糖氧化酶（notatin）的称呼，现已清楚，抗菌性是由于反应生成的H2O2具灭菌作用。提纯物含有2分子的FAD，作为电子受体，除O2外，也能与2，6，二氯酚、靛酚反应。该酶对葡萄糖具有特异性。米氏常数Km也低（10-3M程度），由于能定量地生成H2O2，因此作为D-葡萄糖的定量试剂而广泛被应用于生物化学领域和临床检查。

Glucose oxidase is an enzyme found in molds such as Penicillium notatum and honey. It can catalyze the reaction of d-glucose + O2D-gluconic acid (δ-lactone) + H2O2. Ec1.1.3.4. The enzyme of Penicillium speciosum (p. notatum) has attracted attention because of its obvious antibacterial activity. Therefore, it is also known as glucose oxidase (notatin) , and it is now clear that the antibacterial activity is due to the reaction generated by H2O2 with sterilization. The purified product contains 2-molecule FAD, which can react with 2,6, dichlorophenol and Indophenol besides O 2 as electron acceptor. The enzyme is specific for glucose. The Michaelis constant Km is also low (10-3M degree) and is widely used in biochemistry and clinical examination as a quantitative reagent for d-glucose because of its ability to quantitatively generate H2O2.